Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal binding domain in pRSET-B vector. Recombinant protein was expressed in E. coli cells and soluble fraction...
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| Autor principal: | |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Czech Academy of Agricultural Sciences
2001-02-01
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| Col·lecció: | Veterinární Medicína |
| Matèries: | |
| Accés en línia: | https://vetmed.agriculturejournals.cz/artkey/vet-200102-0005_purification-of-escherichia-coli-expressed-his-tagged-maedi-visna-p25-core-antigen-by-ni2-chelate-affinity-chr.php |
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