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An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

BACKGROUND: Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner. RESULTS: We developed...

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Autori principali: Scheich, Christoph, Sievert, Volker, Büssow, Konrad
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2003
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC183854/
https://ncbi.nlm.nih.gov/pubmed/12885298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-3-12
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