Engineering the l-Arabinose Isomerase from Enterococcus Faecium for d-Tagatose Synthesis

l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal His-tagged-l-AI proteins were produced. The cloning of the two chimeric genes into r...

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Autori principali:	Marylane de Sousa, Ricardo M. Manzo, José L. García, Enrique J. Mammarella, Luciana R. B. Gonçalves, Benevides C. Pessela
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	MDPI AG 2017-12-01
Serie:	Molecules
Soggetti:	">l-arabinose isomerase recombinant DNA affinity purification ">d-tagatose ">d-galactose
Accesso online:	https://www.mdpi.com/1420-3049/22/12/2164
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