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Protocol for sorting cancer stem cells using a combination of anti-CD133 antibody and lectin cyanovirin-N

Summary: CD133 is widely used as a marker to isolate cancer stem cells (CSCs). However, the structural ambiguity of N-glycan of CD133 limits its application in the isolation of CSCs. Here, we present a protocol to sort CSCs from tumor samples by combining CD133 with α-1,2-high-mannose type glycan ch...

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Principais autores: Yuanyan Wei, Shuting Geng, Qihang Chen, Jiayue Chen, Zhijun Fan, Yi Li, Yu Si, Yuerong Yang, Ziwei Liang, Jianhai Jiang
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2024-12-01
Serier:STAR Protocols
Fag:
cancer
cell biology
cell isolation
stem cells
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166724006087
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