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Effect of Sample Preparation on the Detection and Quantification of Selected Nuts Allergenic Proteins by LC-MS/MS

The detection and quantification of nut allergens remains a major challenge. The liquid chroma-tography tandem mass spectrometry (LC-MS/MS) is emerging as one of the most widely used methods, but sample preparation prior to the analysis is still a key issue. The objective of this work was to establi...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Sorel Tchewonpi Sagu, Gerd Huschek, Thomas Homann, Harshadrai M. Rawel
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI AG 2021-08-01
Schriftenreihe:Molecules
Schlagworte:
nut allergenic proteins
sample preparation
protein extraction
tryptic digestion
microwave assisted digestion
SDS PAGE
Online-Zugang:https://www.mdpi.com/1420-3049/26/15/4698
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