The insertion of the inverted repeat of an insertion sequence (IS) element from Lacticaseibacillus rhamnosus changes the host range and stability of pGK12, a shuttle vector for lactic acid bacteria

ABSTRACT Insertion sequences (ISs) are key components of most bacterial genomes and play a crucial role in bacterial mutagenesis. In this study, we observed the insertion of an IS element, ISLrh, from the Lacticaseibacillus rhamnosus M1 genome into plasmid pGK12, resulting in the generation of a new...

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Bibliographic Details

Main Authors:	Zifan Xie, Yong-Su Jin, Todd R. Klaenhammer, Michael J. Miller
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology 2025-04-01
Series:	Applied and Environmental Microbiology
Subjects:	Lactobacillus IS element plasmids plasmid replication heterologous gene expression
Online Access:	https://journals.asm.org/doi/10.1128/aem.01908-24
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