The insertion of the inverted repeat of an insertion sequence (IS) element from Lacticaseibacillus rhamnosus changes the host range and stability of pGK12, a shuttle vector for lactic acid bacteria

ABSTRACT Insertion sequences (ISs) are key components of most bacterial genomes and play a crucial role in bacterial mutagenesis. In this study, we observed the insertion of an IS element, ISLrh, from the Lacticaseibacillus rhamnosus M1 genome into plasmid pGK12, resulting in the generation of a new...

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Detalhes bibliográficos

Principais autores:	Zifan Xie, Yong-Su Jin, Todd R. Klaenhammer, Michael J. Miller
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology 2025-04-01
coleção:	Applied and Environmental Microbiology
Assuntos:	Lactobacillus IS element plasmids plasmid replication heterologous gene expression
Acesso em linha:	https://journals.asm.org/doi/10.1128/aem.01908-24
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