Absolute Quantitation of Specific mRNAs in Cell and Tissue Samples by Comparative PCR
A comparative PCR assay, for the absolute quantitation of specific mRNAs in cell and tissue samples, has been designed to overcome problems with previous techniques. cDNAs made from the RNAs are coamplified with “competitor” plasmid templates under conditions in which reagents are not limiting at th...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Taylor & Francis Group
1999-07-01
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| Schriftenreihe: | BioTechniques |
| Online-Zugang: | https://www.future-science.com/doi/10.2144/99271rr02 |
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