An Optimized RT-qPCR Protocol for Comprehensive Analysis of microRNAs and mRNAs in <i>Mus musculus</i> Brain Tissues

<b>Background/Objectives:</b> MicroRNAs are key post-transcriptional regulators involved in various diseases. Despite its status as the gold standard, real-time RT-PCR faces challenges arising from high sequence homology among closely related microRNAs and the substantial biomaterial required to enr...

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Hauptverfasser:	Maria V. Lukashevich, Margarita M. Rudenok, Suzanna A. Partevian, Maria I. Shadrina, Petr A. Slominsky, Anelya Kh. Alieva
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	MDPI AG 2026-06-01
Schriftenreihe:	Non-Coding RNA
Schlagworte:	microRNA expression analysis method stem-loop real-time PCR total RNA
Online-Zugang:	https://www.mdpi.com/2311-553X/12/3/20
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