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ABC cloning: An efficient, simple, and rapid restriction/ligase-free method

DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives. Here we describe a PCR-based cloning method named ABC cloning. This m...

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Detaylı Bibliyografya
Asıl Yazarlar: Samir El Qaidi, Philip R. Hardwidge
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2019-01-01
Seri Bilgileri:MethodsX
Online Erişim:http://www.sciencedirect.com/science/article/pii/S2215016119300184
Etiketler: Etiketle
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