ABC cloning: An efficient, simple, and rapid restriction/ligase-free method
DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives. Here we describe a PCR-based cloning method named ABC cloning. This m...
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| Principais autores: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2019-01-01
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| Serier: | MethodsX |
| Online adgang: | http://www.sciencedirect.com/science/article/pii/S2215016119300184 |
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