Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS
A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was vali...
Kaydedildi:
| Asıl Yazarlar: | , , , , , , , |
|---|---|
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Elsevier
2015-12-01
|
| Seri Bilgileri: | Journal of Pharmaceutical Analysis |
| Konular: | |
| Online Erişim: | http://www.sciencedirect.com/science/article/pii/S2095177915300022 |
| Etiketler: |
Etiket eklenmemiş, İlk siz ekleyin!
|