Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis

The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amp...

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Autors principals:	Elizabeth Cortez-Herrera, Rosa Dea Sperhacke, Daniela Becker, Afrânio Kritski, Arnaldo Zaha, Maria Lucia Rosa Rossetti
Format:	Artigo
Idioma:	Inglês
Publicat:	Instituto de Tecnologia do Paraná (Tecpar) 2008-08-01
Col·lecció:	Brazilian Archives of Biology and Technology
Matèries:	Polymerase Chain Reaction tuberculosis internal control nucleic acid amplification uracyl DNA glycosilase
Accés en línia:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006
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