Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis

The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amp...

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Bibliografiske detaljer

Principais autores:	Elizabeth Cortez-Herrera, Rosa Dea Sperhacke, Daniela Becker, Afrânio Kritski, Arnaldo Zaha, Maria Lucia Rosa Rossetti
Format:	Artigo
Sprog:	Inglês
Udgivet:	Instituto de Tecnologia do Paraná (Tecpar) 2008-08-01
Serier:	Brazilian Archives of Biology and Technology
Fag:	Polymerase Chain Reaction tuberculosis internal control nucleic acid amplification uracyl DNA glycosilase
Online adgang:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006
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