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Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP

GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors (GEFs). Monitoring the nucleotide exchange in vitro, toge...

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Autors principals: Tomoharu Kanie, Peter Jackson
Format: Artigo
Idioma:Inglês
Publicat: Bio-protocol LLC 2018-04-01
Col·lecció:Bio-Protocol
Accés en línia:https://bio-protocol.org/en/bpdetail?id=2795&type=0
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