Optimized Protocol for the In Situ Derivatization of Glutathione with <i>N</i>-Ethylmaleimide in Cultured Cells and the Simultaneous Determination of Glutathione/Glutathione Disulfide Ratio by HPLC-UV-QTOF-MS

Glutathione (GSH) and glutathione disulfide (GSSG) are commonly used to assess the oxidative status of a biological system. Various protocols are available for the analysis of GSH and GSSG in biomedical specimens. In this study, we present an optimized protocol for the in situ derivatization of GSH...

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Hauptverfasser:	Xueni Sun, Raffaela S. Berger, Paul Heinrich, Ibtissam Marchiq, Jacques Pouyssegur, Kathrin Renner, Peter J. Oefner, Katja Dettmer
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	MDPI AG 2020-07-01
Schriftenreihe:	Metabolites
Schlagworte:	glutathione glutathione disulfide cell culture liquid chromatography UV detection mass spectrometry
Online-Zugang:	https://www.mdpi.com/2218-1989/10/7/292
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