Optimized Protocol for the In Situ Derivatization of Glutathione with <i>N</i>-Ethylmaleimide in Cultured Cells and the Simultaneous Determination of Glutathione/Glutathione Disulfide Ratio by HPLC-UV-QTOF-MS

Glutathione (GSH) and glutathione disulfide (GSSG) are commonly used to assess the oxidative status of a biological system. Various protocols are available for the analysis of GSH and GSSG in biomedical specimens. In this study, we present an optimized protocol for the in situ derivatization of GSH...

Deskribapen osoa

Xehetasun bibliografikoak

Egile Nagusiak:	Xueni Sun, Raffaela S. Berger, Paul Heinrich, Ibtissam Marchiq, Jacques Pouyssegur, Kathrin Renner, Peter J. Oefner, Katja Dettmer
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	MDPI AG 2020-07-01
Saila:	Metabolites
Gaiak:	glutathione glutathione disulfide cell culture liquid chromatography UV detection mass spectrometry
Sarrera elektronikoa:	https://www.mdpi.com/2218-1989/10/7/292
Etiketak:	Etiketa erantsi Etiketarik gabe, Izan zaitez lehena erregistro honi etiketa jartzen!