Manipulating mRNA-binding protein Cth2 function in budding yeast Saccharomyces cerevisiae

Summary: Here, we present a protocol for modulating the function of the Cth2 mRNA-binding protein (RBP) in Saccharomyces cerevisiae. We describe steps to amplify and integrate mutations in Cth2 that affect its stability and function. Next, we detail the functional assay to verify the activity of the...

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Principais autores:	Praveen K. Patnaik, Hanna Barlit, Vyacheslav M. Labunskyy
格式:	Artigo
语言:	Inglês
出版:	Elsevier 2024-03-01
丛编:	STAR Protocols
主题:	Cell-based Assays Model Organisms Molecular Biology Gene Expression
在线阅读:	http://www.sciencedirect.com/science/article/pii/S2666166723007748
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