Manipulating mRNA-binding protein Cth2 function in budding yeast Saccharomyces cerevisiae
Summary: Here, we present a protocol for modulating the function of the Cth2 mRNA-binding protein (RBP) in Saccharomyces cerevisiae. We describe steps to amplify and integrate mutations in Cth2 that affect its stability and function. Next, we detail the functional assay to verify the activity of the...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2024-03-01
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| Collection: | STAR Protocols |
| Sujets: | |
| Accès en ligne: | http://www.sciencedirect.com/science/article/pii/S2666166723007748 |
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