Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors
Summary: Here, we present a protocol for producing 3D pancreatic-like organoids from human pluripotent stem cells in suspension bioreactors. We describe scalable techniques for generating 10,000–100,000 organoids that further mature in 4–5 weeks into α- and β-like cells with glucose-responsive insul...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2023-12-01
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| Col·lecció: | STAR Protocols |
| Matèries: | |
| Accés en línia: | http://www.sciencedirect.com/science/article/pii/S2666166723005476 |
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