Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors

Summary: Here, we present a protocol for producing 3D pancreatic-like organoids from human pluripotent stem cells in suspension bioreactors. We describe scalable techniques for generating 10,000–100,000 organoids that further mature in 4–5 weeks into α- and β-like cells with glucose-responsive insul...

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Bibliografische Detailangaben

Hauptverfasser:	Samuel D. Pollock, Israeli M. Galicia-Silva, Mai Liu, Zoe L. Gruskin, Juan R. Alvarez-Dominguez
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2023-12-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Cell Culture Stem Cells Cell Differentiation Organoids Biotechnology and Bioengineering
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166723005476
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