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Freeze-fracture-etching Electron Microscopy for Facile Analysis of Yeast Ultrastructure

We describe a streamlined method that enables the quick observation of yeast ultrastructure by electron microscopy (EM). Yeast cells are high-pressure frozen, freeze-fractured to cut across the cytoplasm, and freeze-etched to sublimate ice in the cytosol and the organelle lumen. The cellular structu...

Whakaahuatanga katoa

I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Takuma Tsuji, Toyoshi Fujimoto
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Bio-protocol LLC 2017-09-01
Rangatū:Bio-Protocol
Urunga tuihono:https://bio-protocol.org/en/bpdetail?id=2556&type=0
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