A two-photon FRAP protocol to measure the stereociliary membrane diffusivity in rat cochlear hair cells
Summary: Fluorescence recovery after photobleaching (FRAP) has been widely used to monitor membrane properties by measuring the lateral diffusion of fluorescent particles. This protocol describes how to perform two-photon FRAP on the stereocilia of live cochlear inner hair cells using a lipophilic d...
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| Glavni autori: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier
2021-09-01
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| Serija: | STAR Protocols |
| Teme: | |
| Online pristup: | http://www.sciencedirect.com/science/article/pii/S2666166721003440 |
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