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Immunolabeling of Proteins in situ in Escherichia coli K12 Strains

This protocol was developed to label proteins in bacterial cells with antibodies conjugated to a fluorophore for fluorescence microscopy imaging. The procedure is optimized to minimize morphological changes and also to minimize the amount of antibodies needed for the staining. The protocol can also...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Hauptverfasser: Nienke Buddelmeijer, Mirjam Aarsman, Tanneke den Blaauwen
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-protocol LLC 2013-08-01
Schriftenreihe:Bio-Protocol
Online-Zugang:https://bio-protocol.org/en/bpdetail?id=852&type=0
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