Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach
Summary: Here, we describe a protocol for artificially generating hetero-oligomeric protein complexes from the homo-oligomers using a sequential denaturation-renaturation strategy, followed by a modified affinity chromatography protocol used for their purification. This protocol enables one to obtai...
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| Principais autores: | , |
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| 格式: | Artigo |
| 语言: | Inglês |
| 出版: |
Elsevier
2023-03-01
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| 丛编: | STAR Protocols |
| 主题: | |
| 在线阅读: | http://www.sciencedirect.com/science/article/pii/S2666166722009157 |
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