Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis

Summary: Here, we describe a protocol for extraction and tandem immunoprecipitation of the cytoplasmic and nuclear proteins from mouse testis for mass spectrometry. This protocol has been applied to knockin mice that express a meiotic protein of interest tagged with 3xFLAG-HA in the testis. The prot...

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Detalhes bibliográficos

Principais autores:	Naoki Tani, Nobuhiro Tanno, Kei-ichiro Ishiguro
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2022-06-01
Colecção:	STAR Protocols
Assuntos:	Cell Biology Molecular Biology Protein Biochemistry Protein expression and purification Mass Spectrometry
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S266616672200332X
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