Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis
Summary: Here, we describe a protocol for extraction and tandem immunoprecipitation of the cytoplasmic and nuclear proteins from mouse testis for mass spectrometry. This protocol has been applied to knockin mice that express a meiotic protein of interest tagged with 3xFLAG-HA in the testis. The prot...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Elsevier
2022-06-01
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| シリーズ: | STAR Protocols |
| 主題: | |
| オンライン･アクセス: | http://www.sciencedirect.com/science/article/pii/S266616672200332X |
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