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Combining cysteine scanning with chemical labeling to map protein-protein interactions and infer bound structure in an intrinsically disordered region

The Mycobacterium tuberculosis genome harbours nine toxin-antitoxin (TA) systems of the mazEF family. These consist of two proteins, a toxin and an antitoxin, encoded in an operon. While the toxin has a conserved fold, the antitoxins are structurally diverse and the toxin binding region is typically...

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Principais autores: Shahbaz Ahmed, Gopinath Chattopadhyay, Kavyashree Manjunath, Munmun Bhasin, Neelam Singh, Mubashir Rasool, Sayan Das, Varsha Rana, Neha Khan, Debarghya Mitra, Aparna Asok, Ramandeep Singh, Raghavan Varadarajan
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2022-10-01
Serier:Frontiers in Molecular Biosciences
Fag:
chemical labeling
protein:protein interaction
functional residues
evolutionary conservation
structure prediction
mutational scanning
Online adgang:https://www.frontiersin.org/articles/10.3389/fmolb.2022.997653/full
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