Combining cysteine scanning with chemical labeling to map protein-protein interactions and infer bound structure in an intrinsically disordered region

The Mycobacterium tuberculosis genome harbours nine toxin-antitoxin (TA) systems of the mazEF family. These consist of two proteins, a toxin and an antitoxin, encoded in an operon. While the toxin has a conserved fold, the antitoxins are structurally diverse and the toxin binding region is typically...

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Ngā kaituhi matua:	Shahbaz Ahmed, Gopinath Chattopadhyay, Kavyashree Manjunath, Munmun Bhasin, Neelam Singh, Mubashir Rasool, Sayan Das, Varsha Rana, Neha Khan, Debarghya Mitra, Aparna Asok, Ramandeep Singh, Raghavan Varadarajan
Hōputu:	Artigo
Reo:	Inglês
I whakaputaina:	Frontiers Media S.A. 2022-10-01
Rangatū:	Frontiers in Molecular Biosciences
Ngā marau:	chemical labeling protein:protein interaction functional residues evolutionary conservation structure prediction mutational scanning
Urunga tuihono:	https://www.frontiersin.org/articles/10.3389/fmolb.2022.997653/full
Ngā Tūtohu:	Tāpirihia he Tūtohu Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!