The His-tag as a decoy modulating preferred orientation in cryoEM

The His-tag is a widely used affinity tag that facilitates purification by means of affinity chromatography of recombinant proteins for functional and structural studies. We show here that His-tag presence affects how coproheme decarboxylase interacts with the air-water interface during grid prepara...

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Bibliografske podrobnosti

Principais autores:	Raquel Bromberg, Kai Cai, Yirui Guo, Daniel Plymire, Tabitha Emde, Maciej Puzio, Dominika Borek, Zbyszek Otwinowski
Format:	Artigo
Jezik:	Inglês
Izdano:	Frontiers Media S.A. 2022-10-01
Serija:	Frontiers in Molecular Biosciences
Teme:	cryo-electron microscopy single particle reconstruction cryoEM SPR air-water interface AWI His-tag preferred orientation
Online dostop:	https://www.frontiersin.org/articles/10.3389/fmolb.2022.912072/full
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