Hot Fusion: an efficient method to clone multiple DNA fragments as well as inverted repeats without ligase.
Molecular cloning is utilized in nearly every facet of biological and medical research. We have developed a method, termed Hot Fusion, to efficiently clone one or multiple DNA fragments into plasmid vectors without the use of ligase. The method is directional, produces seamless junctions and is not...
Gardado en:
| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Acceso en liña: | http://europepmc.org/articles/PMC4281135?pdf=render |
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