Viral viability markers of SARS-CoV-2: a comparison of cell culture, genomic RNA RT-PCR, and subgenomic RNA RT-PCR

ABSTRACT Accurate methods to assess viral viability are crucial for determining isolation duration and antiviral therapy in immunocompromised patients. Although cell culture (CC) is the gold standard, it has limitations. Cycle threshold (Ct) values from genomic RNA (gRNA) RT-PCR and subgenomic RNA (...

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Detalhes bibliográficos

Principais autores:	Genoveva Cuesta-Chasco, Francesco Tommaso Aiello, Cristina Rodriguez, Anna Villasante, Juan Carlos Hurtado, Francesc Fernández Avilés, Luis Gerardo Rodríguez-Lobato, Mireia Navarro, Ignacio Grafia, Marta Bodro, Carolina García-Vidal, Alex Soriano, Maria Angeles Marcos
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology 2025-10-01
coleção:	Microbiology Spectrum
Assuntos:	subgenomic RNA SARS-CoV-2 surrogate markers of active viral replication
Acesso em linha:	https://journals.asm.org/doi/10.1128/spectrum.00066-25
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