Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells

Summary: Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) throu...

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Detalhes bibliográficos

Principais autores:	Ronghui Li, Juan Carlos Izpisua Belmonte
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2021-09-01
coleção:	STAR Protocols
Assuntos:	Cell Biology Cell culture Developmental biology Stem Cells Cell Differentiation Organoids
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166721004524
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