Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells

Summary: Hematopoietic stem cells (HSCs) generate blood and immune cells. Here, we present a protocol to differentiate human pluripotent stem cells (hPSCs) into hematopoietic progenitors that express the signature HSC transcription factors HLF, HOXA5, HOXA7, HOXA9, and HOXA10. hPSCs are dissociated,...

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Hlavní autoři: Sherry Li Zheng, Jonas L. Fowler, Julie Y. Chen, Christopher Li, Elaine Lin, Alana T. Nguyen, Angela Chen, George Q. Daley, Lay Teng Ang, Kyle M. Loh
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2025-03-01
Edice:STAR Protocols
Témata:
cell differentiation
developmental biology
stem cells
On-line přístup:http://www.sciencedirect.com/science/article/pii/S2666166724007573
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