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An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin. The cell culture conditions have been optimized for inducing expression of keratinocyte differentiation markers. Cells are cultured in the...

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Principais autores: Ratklao Siriwach, Anh Quynh Ngo, Shuh Narumiya, Dean Thumkeo
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2022-12-01
coleção:STAR Protocols
Assuntos:
Sequence analysis
Cell Biology
Single Cell
Developmental biology
RNAseq
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166722007869
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