Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve

Summary: Single-molecule fluorescence in situ hybridization (smFISH) allows spatial mapping of gene expression. This protocol presents advances in smFISH fidelity and flexibility in intact murine sensory nervous system tissue. An approach using RNAscope probes allows multiplexing, enhanced target sp...

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Principais autores:	Xinbei Li, Sreenivas Eadara, Sangmin Jeon, Yan Liu, Gabriella Muwanga, Lintao Qu, Michael J. Caterina, Mollie K. Meffert
格式:	Artigo
語言:	Inglês
出版:	Elsevier 2021-06-01
叢編:	STAR Protocols
主題:	Microscopy Molecular Biology In Situ Hybridization Neuroscience Molecular/Chemical Probes
在線閱讀:	http://www.sciencedirect.com/science/article/pii/S2666166721002628
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