Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve

Summary: Single-molecule fluorescence in situ hybridization (smFISH) allows spatial mapping of gene expression. This protocol presents advances in smFISH fidelity and flexibility in intact murine sensory nervous system tissue. An approach using RNAscope probes allows multiplexing, enhanced target sp...

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Hlavní autoři:	Xinbei Li, Sreenivas Eadara, Sangmin Jeon, Yan Liu, Gabriella Muwanga, Lintao Qu, Michael J. Caterina, Mollie K. Meffert
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2021-06-01
Edice:	STAR Protocols
Témata:	Microscopy Molecular Biology In Situ Hybridization Neuroscience Molecular/Chemical Probes
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2666166721002628
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