Purification of ribosomes from human embryonic stem (hES) cells for high-resolution Cryo-EM structural studies
Human ribosome structures at near-atomic resolution have been determined predominantly from transformed cell lines, often using translation inhibitors during purification that can introduce structural artifacts. We present a robust and scalable protocol for purifying 80S ribosomes from human embryon...
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| Hlavní autoři: | , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
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Frontiers Media S.A.
2026-02-01
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| Edice: | Frontiers in Molecular Biosciences |
| Témata: | |
| On-line přístup: | https://www.frontiersin.org/articles/10.3389/fmolb.2026.1778824/full |
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