pEGFP-E1A真核表达载体的构建及其体外抑制SMMC-7721肝癌细胞作用研究
目的构建含有EGFP及Ad5.EIA基因的重组真核表达载体pEGFP-E1A,研究其在体外对肝癌细胞的裂解作用。方法对pUC119-E1A质粒双酶切获得E1A基因,定向克隆带EGFP和neo筛选标记的真核表达载体到pEGFP-C1载体中,构建pEGFP-E1A质粒,通过脂质体导入人肝癌细胞SMMC-7721中,经RT-PCR及间接免疫荧光方法确认EIA基因的导入,DNAladder法检测EIA基因对SMMC-7721细胞的凋亡诱导作用。结果酶切和序列测定结果显示重组质粒构建正确,RT-PCR及间接免疫荧光结果显示,外源性E1A基因已整合于SMMC-7721细胞并获稳定表达,在6小时就导致SMM...
Wedi'i Gadw mewn:
| Prif Awduron: | , , , |
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| Fformat: | Artigo |
| Iaith: | Chinês |
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Editorial Office of Chinese Journal of Laboratory Diagnosis
2010-01-01
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| Cyfres: | Zhongguo shiyan zhenduanxue |
| Pynciau: | |
| Mynediad Ar-lein: | http://ZSZD.publish.founderss.cn/thesisDetails?columnId=88518688&Fpath=home&index=0 |
| Tagiau: |
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
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