pEGFP-E1A真核表达载体的构建及其体外抑制SMMC-7721肝癌细胞作用研究
目的构建含有EGFP及Ad5.EIA基因的重组真核表达载体pEGFP-E1A,研究其在体外对肝癌细胞的裂解作用。方法对pUC119-E1A质粒双酶切获得E1A基因,定向克隆带EGFP和neo筛选标记的真核表达载体到pEGFP-C1载体中,构建pEGFP-E1A质粒,通过脂质体导入人肝癌细胞SMMC-7721中,经RT-PCR及间接免疫荧光方法确认EIA基因的导入,DNAladder法检测EIA基因对SMMC-7721细胞的凋亡诱导作用。结果酶切和序列测定结果显示重组质粒构建正确,RT-PCR及间接免疫荧光结果显示,外源性E1A基因已整合于SMMC-7721细胞并获稳定表达,在6小时就导致SMM...
I tiakina i:
| Ngā kaituhi matua: | , , , |
|---|---|
| Hōputu: | Artigo |
| Reo: | Chinês |
| I whakaputaina: |
Editorial Office of Chinese Journal of Laboratory Diagnosis
2010-01-01
|
| Rangatū: | Zhongguo shiyan zhenduanxue |
| Ngā marau: | |
| Urunga tuihono: | http://ZSZD.publish.founderss.cn/thesisDetails?columnId=88518688&Fpath=home&index=0 |
| Ngā Tūtohu: |
Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!
|