Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning

Here we present an improved strategy for producing T-cell receptor (TCR)-expressing retroviral vectors using a Golden Gate cloning strategy. This method takes advantage of the modular nature of TCR genes by directly amplifying TCR α and β variable regions from RNA or cDNA, then cloning and fusing th...

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Detalles Bibliográficos

Principais autores:	Lori V. Coren, Sumiti Jain, Matthew T. Trivett, Claes Ohlen, David E. Ott
Formato:	Artigo
Idioma:	Inglês
Publicado:	Taylor & Francis Group 2015-03-01
Series:	BioTechniques
Assuntos:	T-cell receptor Golden Gate cloning retroviral vector gene engineering
Acceso en liña:	https://www.future-science.com/doi/10.2144/000114265
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