Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning
Here we present an improved strategy for producing T-cell receptor (TCR)-expressing retroviral vectors using a Golden Gate cloning strategy. This method takes advantage of the modular nature of TCR genes by directly amplifying TCR α and β variable regions from RNA or cDNA, then cloning and fusing th...
Kaydedildi:
| Asıl Yazarlar: | , , , , |
|---|---|
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Taylor & Francis Group
2015-03-01
|
| Seri Bilgileri: | BioTechniques |
| Konular: | |
| Online Erişim: | https://www.future-science.com/doi/10.2144/000114265 |
| Etiketler: |
Etiket eklenmemiş, İlk siz ekleyin!
|