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Continuous, label-free, 96-well-based determination of cell migration using confluence measurement

Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicabi...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Christian Mayr, Marlena Beyreis, Heidemarie Dobias, Martin Gaisberger, Julia Fuchs, Martin Pichler, Markus Ritter, Martin Jakab, Katharina Helm, Daniel Neureiter, Tobias Kiesslich
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis Group 2019-01-01
Schriftenreihe:Cell Adhesion & Migration
Schlagworte:
migration
gap-closure assay
confluence measurement
non-endpoint
high-throughput
continuous measurement
cytochalasin d
ouabain
epidermal growth factor
a549 human lung carcinoma cells
Online-Zugang:http://dx.doi.org/10.1080/19336918.2018.1526612
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