Protocol for measuring cell cycle Zn2+ dynamics using a FRET-based biosensor
Summary: The exchangeable Zn2+ pool in cells is not static but responds to perturbations as well as fluctuates naturally through the cell cycle. Here, we present a protocol to carry out long-term live-cell imaging of cells expressing a cytosolic Zn2+ sensor. We then describe how to track cells using...
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| Principais autores: | , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier
2024-06-01
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| 叢編: | STAR Protocols |
| 主題: | |
| 在線閱讀: | http://www.sciencedirect.com/science/article/pii/S2666166724002879 |
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