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Protocol for measuring cell cycle Zn2+ dynamics using a FRET-based biosensor

Summary: The exchangeable Zn2+ pool in cells is not static but responds to perturbations as well as fluctuates naturally through the cell cycle. Here, we present a protocol to carry out long-term live-cell imaging of cells expressing a cytosolic Zn2+ sensor. We then describe how to track cells using...

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Ngā kaituhi matua: Samuel E. Holtzen, Ananya Rakshit, Amy E. Palmer
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Elsevier 2024-06-01
Rangatū:STAR Protocols
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Urunga tuihono:http://www.sciencedirect.com/science/article/pii/S2666166724002879
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