tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the moss Physcomitrella
Abstract CRISPR/Cas9-based genome editing in the model bryophyte Physcomitrium patens (commonly known as Physcomitrella) is widely used for gene knockout via small insertions or deletions (indels). In this study, we developed an efficient dual-gRNA system capable of producing large, targeted deletio...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BMC
2026-02-01
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| Colecção: | Plant Methods |
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1186/s13007-026-01509-6 |
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