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tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the moss Physcomitrella

Abstract CRISPR/Cas9-based genome editing in the model bryophyte Physcomitrium patens (commonly known as Physcomitrella) is widely used for gene knockout via small insertions or deletions (indels). In this study, we developed an efficient dual-gRNA system capable of producing large, targeted deletio...

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I tiakina i:
Ngā taipitopito rārangi puna kōrero
Kaituhi matua: Elena Kozgunova
Hōputu: Artigo
Reo:Inglês
I whakaputaina: BMC 2026-02-01
Rangatū:Plant Methods
Ngā marau:
Urunga tuihono:https://doi.org/10.1186/s13007-026-01509-6
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