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GC-MS Discrimination of Citrulline from Ornithine and Homocitrulline from Lysine by Chemical Derivatization: Evidence of Formation of <i>N</i><sup>5</sup>-Carboxy-ornithine and <i>N</i><sup>6</sup>-Carboxy-lysine

Derivatization of amino acids by 2 M HCl/CH<sub>3</sub>OH (60 min, 80 °C) followed by derivatization of the intermediate methyl esters with pentafluoropropionic anhydride (PFPA) in ethyl acetate (30 min, 65 °C) is a useful two-step derivatization procedure (procedure A) for their quantitative measur...

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Principais autores: Svetlana Baskal, Alexander Bollenbach, Dimitrios Tsikas
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2021-04-01
coleção:Molecules
Assuntos:
amino acids
derivatization
esterification
GC-MS
pentafluoropropionic anhydride
ureide
Acesso em linha:https://www.mdpi.com/1420-3049/26/8/2301
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