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Diazirine-functionalized mannosides for photoaffinity labeling: trouble with FimH

Photoaffinity labeling is frequently employed for the investigation of ligand–receptor interactions in solution. We have employed an interdisciplinary methodology to achieve facile photolabeling of the lectin FimH, which is a bacterial protein, crucial for adhesion, colonization and infection. Follo...

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Principais autores: Femke Beiroth, Tomas Koudelka, Thorsten Overath, Stefan D. Knight, Andreas Tholey, Thisbe K. Lindhorst
Format: Artigo
Sprog:Inglês
Udgivet: Beilstein-Institut 2018-07-01
Serier:Beilstein Journal of Organic Chemistry
Fag:
diazirines
docking
FimH
lectin ligands
mannosides
mass spectrometry
photoaffinity labelling
Online adgang:https://doi.org/10.3762/bjoc.14.163
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