A rapid ceramide synthase activity using NBD-sphinganine and solid phase extraction

Ceramides are synthesized by six mammalian ceramide synthases (CerSs), each of which uses fatty acyl-CoAs of different chain lengths for N-acylation of the sphingoid long-chain base. We now describe a rapid and reliable CerS assay that uses a fluorescent N-[6-[(7-nitrobenzo-2-oxa-1,3-diazol-4-yl) (N...

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Hlavní autoři:	Rotem Tidhar, Kacee Sims, Eden Rosenfeld-Gur, Walter Shaw, Anthony H. Futerman
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2015-01-01
Edice:	Journal of Lipid Research
Témata:	ceramides sphingolipids in vitro assay chromatography enzymology long-chain base
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S0022227520356522
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